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Cellect™

Monoclonal antibodies

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Ideal for human embryonic & induced pluripotent stem cell research

High quality antibodies against cell surface antigens. Ideally suited for the identification, characterization and isolation of undifferentiated hES and hiPS cells.

The Cellect™ range of monoclonal antibodies has been specifically developed for pluripotent stem cell research. The antibodies recognize cell surface antigens on undifferentiated human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells which are down-regulated during differentiation. hES-Cellect™ and ESCellect™ are ideally suited for the characterization and isolation of human pluripotent stem (hPS) cells as well as separation of hES cells from feeder cells or differentiated progeny using magnetic cell separation or flow sorting. The antibodies facilitate the identification of newly reprogrammed hiPS cell clones using non-cytotoxic live cell staining. hES-Cellect™ is specific for human pluripotent cells, while ES-Cellect™ can be employed in human as well as mouse stem cell research. hFF-Cellect™ is a reliable surface antibody developed for the characterization and depletion of human feeder cells or identification of non-reprogrammed fibroblasts during hiPS cell derivation.

Figure 1: Flow cytometric quantification

 

Figure 1: Flow cytometric quantification of undifferentiated hES cells using hES-Cellect™ (red) and ES-Cellect™ (green) compared to SSEA-1 (blue) and TRA 1-60 (purple).

 
Figure 2: Undifferentiated human pluripotent stem cells   Figure 3: Early hiPS cell clones identified with hES-Cellect™non-cytotoxic surface staining.
Figure 2: Undifferentiated human pluripotent stem cells in Cellartis feeder free DEF™-CS (culture system) characterized by ES-Cellect™ immunocytochemical staining in green (A), nuclei stained in blue (B).   Figure 3: Immunocytochemistry staining of mouse ES cells on mouse feeder cells using ES-Cellect™

Applications

  • Immunocytochemistry, flow cytometry and magnetic cell separation
  • Identification of hES & hiPS cells
  • Separation of hES & hiPS cells from feeder cells and differentiated progeny

Advantages

  • Allow early identification of reprogrammed hiPS cell clones
  • Cell surface markers
  • Do not require cell fixation or permeabilization
  • Non-cytotoxic for the detection and selection of viable cells
  • Reduced cross reactivity compared to SSEA and TRA antibodies against fibroblasts and other human somatic cells

 

PRODUCT CAT. # ANTIBODY SPECIFICITY PURITY
MAB-801-VIAL hES-Cellect™ Undiff. hES & hiPS cells >95%
MAB-802-VIAL ES-Cellect™ Undiff. hES & hiPS cells & murine pluripotent stem cells >95%
MAB-803-VIAL hFF-Cellect™ Human fibroblasts >95%

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