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SCED™ 461

Human ES cells

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SCED™461 is an undifferentiated human embryonic stem (hES) cell line for propagation by enzymatic dissociation into single cells.

Propagation by single-cell enzymatic dissociation enables a robust cell culture system for scale-up of hES cell culture. SCED™461 has a great advantage over the manual dissection method of hES cell culture as no special equipment or training is required.

A population of undifferentiated human embryonic stem cells in single cell suspension. Cells are provided frozen in medium containing FBS and DMSO. SCED™461 cells provide a robust, easy to use cell culture system for hES cell research.

 
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Figure 1: SCED™ 461 cells stained for Oct-4   Figure 2: SCED™461 cell colony on Human foreskin fibroblast feeders, 2 days after passage
 

Applications

SCED™461 cells are ideally suited to scientists new to human embryonic stem cell (hES) culture and also laboratories that require efficient scale up of hES. The cells can be used in research applications such as:

  • Differentiation induction studies
  • Fluorescence activated cell sorting (FACS)
  • hES scale up for automation
  • Clonal selection

Advantages

  • Easy to culture compared to traditional hES cell culture
  • No special equipment or training required
  • Extremely robust culture system

Table of Characteristics
Morphology Undifferentiated
Immunocytochemistry
Oct-4, TRA1-60, TRA1-81, SSEA-3, SSEA-4, Alkaline Phosphatase Positive
SSEA-1 Negative
qRT-PCR analysis
Oct-4, Nanog, Cripto Positive
α-fetoprotein Negative
 
Karyotype 46 XY
FISH 2nXY
Pluripotency
(Teratoma – tissues formed)		 Endodermal
Mesodermal
Ectodermal

REFERENCES

Ellerström C, Strehl R, Noaksson K, Hyllner J, Semb H, Facilitated Expansion of Human Embryonic Stem Cells by Single-Cell Enzymatic Dissociation,  Stem Cells Jul 2007; 25: 1690 - 1696

Ellerström C, Hyllner J and Strehl R, 2010, Single cell enzymatic dissociation of human embryonic stem cells: a straightforward, robust, and standardized culture method, Methods Mol Biol, 584:121-34

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