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Mesenchymal Progenitors

hES-MP™ 002.5

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Mesenchymal progenitors derived from hESC, a homogeneous and robust source for in vitro use in research applications

hES-MP™002.5 are hES cell-derived mesenchymal progenitors proven to have a high resemblance to adult human mesenchymal stem cells (hMSCs). The cells can be differentiated towards several mesodermal lineages. The hES-MP™002.5 are specifically efficient for the differentiations towards the osteogenic lineage and have a high mineralisation potential after protracted culture. The cells are delivered frozen in a single cell suspension.

The hES-MP™002.5 are an ideal platform for bulk production of mesenchymal cells where a homogeneous, robust and unlimited source of cells is needed.

 
Cell doublings of hMSCs compared to hES-MP™00.5

Figure 1: Cell doublings of hMSCs compared to hES-MP™00.5

Hierarchical clustering of genes with an FC ≥ 20

Figure 2: Hierarchical clustering of genes with an FC ≥ 20, showing a similar gene expression pattern in hES-MP™002.5 and hMSCs
 

Advantages

  • A homogeneous, LOT-tested cell population with no donor to donor variability in the cell population
  • Robust and easy to culture, accelerated scale up for multiple experiments
  • High resemblance to adult human mesenchymal stem cells (hMSCs)
  • Ability to differentiate towards tissues of the mesodermal lineages
  • High mineralization potential after protracted expansion

Applications

  • Mesenchymal induction and differentiation studies
  • Tissue engineering models for repair and regeneration

Table of Characteristics
Mesodermal differentation
Osteogenic differentiation positive von Kossa staining (mineralisation)
Chondrogenic differentiation positive Alcain blue van Gieson and safranin-O staining
Adipogenic differentiation positive oil-red O staining
Flow cytometry analysis
CD166 +
CD105 +
CD13 +
CD10 +
CD133 -
CD117 -
PRODUCT CATALOGUE # SOURCE
hES-MP™002.5 MPC-301-VIAL hESC line SA002.5, a subclone to SA002 registered by European hESC registry and by NIH

REFERENCES

1. de Peppo GM et al,, Osteogenic potential of human mesenchymal stem cells and human embryonic stem cell-derived mesodermal progenitors: a tissue engineering perspective. Tissue Eng Part A.. 2010 Nov;16(11):3413-26.

2. de Peppo GM et al, Human embryonic mesodermal progenitors highly resemble human mesenchymal stem cells and display high potential for tissue engineering applications. Tissue Eng Part A.2010 Jul

3. Emanuelsson K and Karlsson C etal, Human embryonic stem cell-derived mesenchymal progenitors-Potential in regenerative medicine. Stem Cell Res.2009 May 19

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