Hepatocytes

hES-HEP™ - Hepatocyte-like cells

hepatocyte-like_cells_prodimg

Fresh hepatocyte-like cells for in vitro use in hepatocyte research applications

hES-HEP™ is a highly homogeneous population of hES cell derived hepatocytes. These cells are shipped fresh, ready-to-use in 6-, 24- and 96 well plate formats. The cells are ideal for use in hepatocyte related applications that demand a highly reproducible platform and continuous supply of material.

Data from several global expression profiling experiments from hES cell derived hepatocytes are available for our customers via our Cellartis Customer Login.

Advantages

Human cells
More than 80% homogeneity
High reproducibility
2D-cultures
Multiwell plate format
Freshly delivered, ready-to-use
CYP1A and 3A activity

Example of Applications

Drug discovery
Vaccine development
Toxicology testing

Figure 1: Morphology of hES-HEP™: Polygonal, single- or bi-nucleated cells containing light nuclei with distinct nucleoli.

Figure 2: hES-HEP™ express Hepatocyte nuclear factor 4a (HNF4a), a1-antitrypsin (A1AT), Glutathione S-transferase A1 (GSTA1), and store glycogen as indicated by PAS

Table of Characteristics
Morphology	Polygonal cell shape, nuclei with distinct nucleoli, often bi- or multi-nucleated.
Protein Markers	FOXA2
	HNF4A
	A1AT
	CK18
	CK8
	GSTA1
Gene Expression	CYP1A1, 1A2
	CYP3A4, 3A5
	CYP2C9
	GSTA1
	UGT2B7
	MRP2
	BSEP
	OCT-1
	A1AT
	TAT
Functionality	Glycogen storage
Functionality	CYP1A and CYP3A activity

PRODUCT	CATALOGUE #	SOURCE/FORMAT
hES-HEP™	HEP-102-0006	hESC SA181 / 6 well plate
	HEP-102-0024	hESC SA181 / 24 well plate
	HEP-102-0096	hESC SA181 / 96 well plate

REFERENCES

1. Yildirimman R et al, Human embryonic stem cell derived hepatocyte-like cells as a tool for in vitro hazard assessment of chemical carcinogenicity. Toxicol Sci. 2011 Aug 27. [Epub ahead of print]

2. Brolén G et al, Hepatocyte-like cells derived from human embryonic stem cells specifically via definitive endoderm and a progenitor stage, J Biotechnology.
2010, 145:284-294.

3. Jensen, J et al, Human embryonic stem cell technologies and drug discovery J Cell Physiol.
2009 Jun;219(3):513-9.

4. Heins N et al, Stem Cells 2004;22:367-376
United States National Stem Cell Bank; http://www.nationalstemcellbank.org

5. Mantel N et al, Potential markers of attenuation of YF virus after infection of stem cell-derived human hepatocytes with wild-type Asibi or live-attenuated YF 17D virus, Supplement to the American Journal of tropical Medicine and Hygiene.
Volume 83, November 2010, Number 5, abstract 12.

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