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Cardiomyocyte monolayers

hES-CMC™2D

cardiomyocyte_monolayers_prodimg

Fresh cardiomyocytes, ready for in vitro use and easily dissociated for single cell applications

hES-CMC™2D are hES cell-derived cardiomyocyte clusters prepared as monolayers in a 96-well plate format. These spontaneously beating cultures exhibit electrical coupling as demonstrated by their synchronous contractions. hES-CMC™2D are well characterised and express cardiomyocytes markers. The hES-CMC™2D are shipped fresh and ready-to-use. hESCMC™2D are available from two different cell lines, SA002 and SA121, both lines registered at the European hESC registry.

Data from several global expression profiling experiments from the hES cell derived cardiomyocytes are available for our customers via the Cellartis Customer Login.

Applications

  • Safety pharmacology
  • Cardiotoxicity testing
  • Discovery of novel cardiac drug targets
  • Research on cardiomyocyte function and development
  • Transfection
  • High Content Analysis

Typical Assays

  • Micro Electrode Array (MEA)
  • Voltage-clamp studies
  • Patch Clamp
  • Ion channel trafficking
  • cTroponin release assays

Advantages

  • Human cells
  • Homogeneous source
  • Native environment of multiple ion channels
  • High toxicological relevance
  • Genetically unmodified
  • Unlimited supply
  • Fresh and ready to use
Figure 1: Schematic illustration of an in vitro testing platform

Figure 1: Schematic illustration of an in vitro testing platform for hES-CMC™2D (adapted from Mandenius et al, 2011)

Table of Characteristics
Cardiac gene
expression		 MYH6
MYH7
ACTC1
TNNT2
Cardiac ion channel
expression		 hERG channel
Voltage gated
potassium channels
Release of cardiac stress bio markers cTroponin
PRODUCT CATALOGUE # SOURCE
hES-CMC™2D CMC-201-002D hES cell line SA002
CMC-202-002D hES cell line SA121

REFERENCES

1. Andersson H et al, Assaying Cardiac Biomarkers for Toxicity Testing using Biosensing and Cardiomyocytes Derived from Human Embryonic Stem Cells.
 J Biotechnol. 2010 Oct 1;150(1):175-81.

2. Asp J et al, Cardiomyocyte Clusters Derived from Human Embryonic Stem Cells Share Similarities with Human Heart Tissue.
 J Mol Cell Biol. 2010 Oct;2(5):276-83.

3. Synnergren J et al, Expression of microRNAs and their target mRNAs in human stem cell derived cardiomyocyte clusters and in heart tissue.
 Physiol Genomics, Sep 14, 2010 (Epub ahead of print).

4. Jonsson MKB et al, Quantified Proarrhythmic Potential of Selected Human Embryonic Stem Cell-derived Cardiomyocytes.
 Stem Cell Res, 2010 May;4(3):189-200.

5. Synnergren J et al, Molecular signature of cardiomyocyte clusters derived from human embryonic stem cells.
 Stem Cells. 2008 Jul;26(7):1831-40.

6. Norström A et al, Molecular and pharmacological properties of human embryonic stem cell derived cardiomyocytes.
 Exp Biol Med. 2006 Dec;231(11):1753-62.

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